LOCUS       40924_5204       11005 bp DNA     circular SYN 17-DEC-2018
DEFINITION  PiggyBac transformation vector pB-UGateway w+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     A toolkit for transformation and mutagenesis in Drosophila using 
            piggyBac
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2002) Invertebrate Targets, Syngenta 
            Biotechnology, Inc., 3054 Cornwallis Rd, Research Triangle Park, NC 
            27709, USA
REFERENCE   3  (bases 1 to 11005)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2002) Invertebrate Targets, Syngenta Biotechnology, Inc., 
            3054 Cornwallis Rd, Research Triangle Park, NC 27709, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(11..620)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     protein_bind    646..740
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        759..997
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    1006..1130
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1155..1185
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1239..1895
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2240..2542
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2586..2710)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          2726..3040
                     /number=3
                     /note="RpS5"
     protein_bind    complement(3089..3113)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3271..3404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3441..3457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3465..3481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3489..3519)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3534..3555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     gene            3579..7698
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     mobile_element  complement(7698..8147)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     promoter        complement(8165..8183)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8204..8220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8228..8244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8252..8282)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8297..8318)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8606..9194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9368..10225)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10226..10330)
                     /label=AmpR promoter
     rep_origin      complement(10356..10811)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     10953..10969
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10979..10997
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctcttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
       61 aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga
      121 tattaagttt tattatattt acacttacat actaataata aattcaacaa actatttatt
      181 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
      241 aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca
      301 tgttgtatta taaaataagt aattagctta acttatacat aatagaaaca aattatactt
      361 attagtcagt cagaaacaac tttggcacat atcaatatta tgctctcgac aaataacttt
      421 tttgcatttt ttgcacgatg catttgcctt tcgccttatt ttagaggggc agtaagtaca
      481 gtaagtacgt tttttcgtta ctggctcttc agtactgtca tctgatgtac caggcacttc
      541 atttggcaaa atattagaga tattatcgcg caaatatctc ttcaaagtag gagcttctaa
      601 acgcttacgc ataaacgatg acgtcccgcg ggcatgcctg caggtcggag tactgtcctc
      661 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
      721 gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa tagaggcgct
      781 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
      841 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta
      901 aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg
      961 ccaagaagta attattgaat acaagaagag aactctgcgg ccatcacaag tttgtacaaa
     1021 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     1081 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     1141 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     1201 aggatccggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1261 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1321 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1381 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1441 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1501 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1561 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1621 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1681 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1741 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1801 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     1861 tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa
     1921 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     1981 atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac
     2041 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     2101 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     2161 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     2221 gagaacaggg actggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     2281 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     2341 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
     2401 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     2461 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     2521 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     2581 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     2641 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     2701 acaaagtggt gatggccgct ctagaactag taggtgggtt tcttctgaag atttgccttc
     2761 cagatgcatg tgctcacggt ggccggtagc aatggcttgt ggttcgttta gagactgctt
     2821 gcttataaca cttggccaga acgaaaatct atctaatatc cgtagcgtca taatgtgtag
     2881 tatagccgac atttggcgta tgaaacgaag agacattatc aattgcataa cacactttca
     2941 ttttactaac gataagaacc ccaaattctt acgctgctgt gattgcgttc ataaagttac
     3001 ttaattaatt tttttccgta tatttccttt aggcactagt ggatcccccg ggctgcagga
     3061 attcgatatc aagcttttta cgtttctcgt tcagcttttt tgtacaaact tgtgatagct
     3121 tgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc cgccttctat
     3181 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     3241 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     3301 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3361 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     3421 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3481 attccacaca ccatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     3541 agctaactca cattaattgc gttgcgctca ctctcgagct agtatgtatg taagttaata
     3601 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
     3661 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
     3721 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     3781 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     3841 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg
     3901 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
     3961 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
     4021 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     4081 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
     4141 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
     4201 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt
     4261 gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat
     4321 atcatgatca agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata
     4381 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
     4441 tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta
     4501 gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc
     4561 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg
     4621 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
     4681 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
     4741 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
     4801 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
     4861 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
     4921 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
     4981 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
     5041 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
     5101 ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc
     5161 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
     5221 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
     5281 cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg
     5341 gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
     5401 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
     5461 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
     5521 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct
     5581 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
     5641 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
     5701 atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc
     5761 aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
     5821 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
     5881 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
     5941 aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac
     6001 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa
     6061 tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
     6121 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata
     6181 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
     6241 taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac tttctaatga
     6301 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
     6361 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt
     6421 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
     6481 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
     6541 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
     6601 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
     6661 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
     6721 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
     6781 tgccccagtt cgggcaaggt catcccggag acgcttaact tctccgccgc cgatctgccg
     6841 ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
     6901 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
     6961 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
     7021 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
     7081 cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc
     7141 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
     7201 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
     7261 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
     7321 cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt
     7381 catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat
     7441 atatttgtat atatttccct cacagctata tttattctaa tttaatatta tgacttttta
     7501 aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca
     7561 tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt
     7621 ttatatatat caaaaataac aactataata ataagaatac atttaattta gaaaatgctt
     7681 ggatttcact ggaactagac gtcatcttca cttacgtgat ctgatatttc actgtcagaa
     7741 tcctcaccaa caagctcgtc atcgctttgc agaagagcag agaggatatg ctcatcgtct
     7801 aaagaactac ccattttatt atatattagt cacgatatct ataacaagaa aatatatata
     7861 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
     7921 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
     7981 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
     8041 gagatgtcct aaacgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
     8101 aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaaggta cccagctttt
     8161 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     8221 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8281 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8341 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8401 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9121 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9181 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9241 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9301 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9361 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9421 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9481 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9541 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9601 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9661 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9721 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9781 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9841 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9901 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9961 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10021 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10081 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10141 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10201 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10261 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10321 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
    10381 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
    10441 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
    10501 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
    10561 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
    10621 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
    10681 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
    10741 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
    10801 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    10861 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    10921 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
    10981 atacgactca ctatagggcg aattg
//
